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2006-10-23: Human SCINT Session in CODATA06 - 6
Poster Mihoko Otake  Registed 2006-11-04 22:18 (2651 hits)

Date: 2006.10.23 (Mon) 15:40-15:55
Place: The China Resources Hotel Beijing
Session: Human Science Integration and its Synthetic Research Methodology (2)
Speaker: Tomohiko Ozaki
Title: Cardiac cells in vitro -contruction and intracellular Ca dynamics
Keywords: Microelectrode arrays, intracellular calcium, cultured cardiomyocyte, Japan

Authors: Tomohiko Ozaki
Affiliation: The University of Tokyo

Bibliography: Ozaki T., Morimoto K., Kurashima T., Takayama Y., Jimbo Y., Cardiac cells in vitro -contraction and intracellular Ca dynamics-. In Proceedings of the 20th CODATA International Conference, pp. 57, 2006.(pdf)

To investigate the relationship between contraction and intracellular Ca dynamics of cadiac cells, we tried simultaneous recording of intracellular Ca transients and spontaneous electrical activity in rat cardiomyocytes cultured on microelectrode arrays (MEAs) with 64 embedded electrodes. The MEA method is one of the promising tools to visualize network activity-patterns. We used the 2 or 3 days old Wistar rat cardiomyocytes and incubeted them in a CO2 incubator at 37℃.To record intracellular Ca dynamics, we used fluo-4 as the indicator. The wavelength of excitation light source was 490nm. We recorded the serial image of the fluorescence of cardiomyocytes at 250 x 250 pixels resolution, 10frame/s, by charge-coupled device camera. Action potentials were measured by 64 microelectrodes. Electrical signals were recorded by 25kHz. In cardiomyocytes at 3DIV or later, synchronized electrical activity and Ca transients were observed, which suggested strong relationship between electrical activity and intracellular Ca dynamics.

[1] Stephan Rohr, André G. Kléber, and Jan P. Kucera : "Optical Recording of Impulse Propagation in Designer Cultures Cardiac Tissue Architectures Inducing Ultra-Slow Conduction", TCM Vol. 9, No. 7, 173/179, (1999)

[2] Norbert Klauke, Godfrey L. Smith,y and Jon Cooper : "Stimulation of Single Isolated Adult Ventricular Myocytes within a Low Volume Using a Planar Microelectrode Array", Biophysical Journal Volume 85, 1766/1774, (2003)

[3] Noriko Inoue, Tomoko Ohkusa, Tomoko Nao, Jong-Kook Lee, Tomo Matsumoto, Yuji Hisamatsu, Takashi Satoh, Masafumi Yano, Kenji Yasui, Itsuo Kodama, Masunori Matsuzaki : "Rapid Electrical Stimulation of Contraction Modulates Gap Junction Protein in Neonatal Rat Cultured
Cardiomyocytes Involvement of Mitogen-Activated Protein Kinases and Effects of Angiotensin II-Receptor Antagonist", Journal of the American College of Cardiology Vol. 44, No. 4, 914/922, (2004)

[4] Günter Wrobel, Chi-Kong Yeung, Frank Sommerhage, Mansun Chan, Andreas Offenhäusser, Sven Ingebrandt : "Validation of the possible use of microelectrode arrays in pharmacological studies" , 5th Int. Meeting on Substrate-Integrated Microelectrodes, 115/116,(2006)

[5] Igor R. Efimov, Vladimir P. Nikolski, Guy Salama : Optical Imaging of the Heart, Circ. Res. 2004;95;21/33,(2004)

[6] DS Rosenbaum and J Jalife: Optical mapping of cardiac excitation and arrhythmias. Future Publishing Company Inc., (2001)

[7] Frank Sommerhage*, Mathias Schindler, Günter Wrobel, Sven Ingebrandt, Andreas Offenhäusser : Simulation of extracellular recorded cardiac action potentials, 5th Int. Meeting on Substrate-Integrated Microelectrodes, 113/114,(2006)

[8] H. Lohmann, A. Bussek, M. Meier, M. Schmidt, E. Wettwer, U. Ravens: MEA recordings from acute heart slices from adult rats and guinea pigs, 5th Int. Meeting on
Substrate-Integrated Microelectrodes, 111/112,(2006)
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